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Electrophysical monitoring of culture process of recombinant Escherichia coli strains

✍ Scribed by Victor D. Bunin; Alexander G. Voloshin; Zoya F. Bunina; A. Vladimir Shmelev

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
505 KB
Volume
51
Category
Article
ISSN
0006-3592

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✦ Synopsis

A novel method for monitoring t h e cell culture process has been developed. The method is based on the measurements of electro-optical characteristics of cell suspension, calculation of cell structure parameters, and the relationship between accumulation of proteins and change of these parameters' employment. Application of the method for the monitoring of a culture process of a recombinant strain is considered. The process of growth of recombinant strains cannot be sufficiently predicted and the direct measurement of cell culture parameters is unlikely to be the most efficient way of solving the problem.

fscherichia coli plasmid-free and recombinant strains synthesizing the fusion protein consisting of tumor necrosis factor-a (TNF) and thymosin-a, (T) were studied. It was found that cytoplasmic electroconductivity of the strains investigated increased during the culture process. The accumulation of insoluble recombinant pThy-315encoded hybrid protein TNF-T in cells resulted in a decrease of the membrane dielectric permeability. To determine variations of membrane dielectric permeability the amount of insoluble recombinant protein TNF-T in the bacterial cells should be calculated.

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