Electrophoretic transport of solutes in aqueous two-phase systems
β Scribed by Mark L. Levine; Milan Bier
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 870 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0173-0835
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π SIMILAR VOLUMES
Two-polymer aqueous phase systems are described in which ribosomes selectively partition into one of the phases. One of the phase systems is used to determine rapidly and conveniently binding of thiostrepton and erythromycin to Escherichia co/i ribosomes under equilibrium conditions.
Phase diagram data at 4Β°C was determined for the aqueous two-phase systems composed of polyethylene glycol, dextran, and water. The Flory-Huggins theory of polymer thermodynamics was used to correlate partitioning of biomolecules in these aqueous two-phase systems resulting in a simple linear relati