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Electrophoretic separation of the plus and minus strands of rotavirus SA11 double-stranded RNAs

โœ Scribed by John T. Patton; Sandrina Stacy-PhippsA


Book ID
119114344
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
566 KB
Volume
13
Category
Article
ISSN
0166-0934

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A simple method for separation of ssRNA from dsRNA on hydroxyapatite is described. The following procedure can be usefully applied for RNA fractionation: (i) Elution of column with 0.1 M phosphate at 60" removes nueleotidrs and a sm:ilI amount of ssRNA. (ii) Elution with 0.1 31 phosphate at 90" remo