๐”– Bobbio Scriptorium
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Electrophoretic separation of nucleic acids: Evaluation by video and photographic densitometry

โœ Scribed by Dr. Steven E. Freeman; Lyndon L. Larcom; Bryan D. Thompson

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
870 KB
Volume
11
Category
Article
ISSN
0173-0835

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โœฆ Synopsis

The separation of D N A by gel electrophoresis provides arapid method for determining size distributions of D N A in solution. Densitometric scanning of photographs of gels has been the standard method of analysis of agarose gels. However, analysis of photographs is complicated by the non-linear response of photographic film. Charged-coupled device video cameras have become popular for quantitative densitometry and we have used a charge-coupled device camera to image agarose gels to quantitate DNA damage. We compare video and photographic densitometry for quantitation of ultraviolet radiation (UV)-induced D N A damage and find that the two methods give equivalent results.

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