Mathematical description of electrophoretic separation of weak acids by means of an H+ pulse

✍ Scribed by Petr Gebauer; Mirko Deml; Jan Pospichal; Dr. Petr Boček

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 635 KB
• Volume: 11
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/elps.1150110909

✦ Synopsis

Abstract

The paper gives a detailed theoretical description of the recently published principle of separation by a dynamic pulse in zone electrophoresis. The theory is illustrated by using a system in which weak acids are separated by migrating against an H^+^ pulse. The mathematical description pertains to (i) the evolution and migration of the concentration profile of the H^+^ pulse, and (ii) the migration of a sample substance (here a weak acid) against and through the pulse. As a result the migration (detection) times of the separated sample components are obtained. Based on the theory, a graphical treatment was developed which allows fast optimization of the separation conditions by observing (on the PC monitor) how the trajectories of the substances (drawn in a time vs. distance coordinate system) change with a change in the parameters of the pulse. Some of the results illustrate the possible advantages of using the dynamic pulse method in analytical practice.