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Electrophoretic separation of Aγ and Gγ human globin chains in nonidet P-40

✍ Scribed by Angelo Guerrasio; Giuseppe Saglio; Umberto Mazza; Piergiorgio Pich; Clara Camaschella; Giuseppe Ricco; Elisabetta Gianazza; Pier Giorgio Righetti


Book ID
115822584
Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
377 KB
Volume
99
Category
Article
ISSN
0009-8981

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## Abstract We cultured human adult peripheral and umbilical cord blood mononuclear cells in methylcellulose and measured the synthetic rates of globin chains in individual erythropoietic bursts. Globin chains were labeled with ^14^C‐amino acids in culture, separated by isoelectric focusing in slab