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Electrophoretic karyotype of cellulolyticPenicillium janthinellumstrains

✍ Scribed by Torsten Kayser; Günter Schulz

Publisher
Springer-Verlag
Year
1991
Tongue
English
Weight
525 KB
Volume
20
Category
Article
ISSN
0172-8083

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✦ Synopsis

The genome of the cellulolytic fungus Penicillium janthinellum BIOURGE was resolved completely by rotating field electrophoresis. The gel pattern revealed 8-10 different chromosomes. On the basis of data for yeast chromosome size standards from Schizosaccharomyces pombe and Saccharomyces cerevisiae, chromosome sizes in the range from 2.0 to 8 mb were estimated. By Southern hybridization with heterologous probes, the chromosomal locations of rDNA, the elongation factor EF1, actin and ubiquitin have all been mapped. In addition, first results with heterologous and homologous probes for cellulolytic genes from Trichoderma reesei and P. janthinellum are presented. In contrast to the other probes, signals obtained with cellulase genes are clearly distributed over several chromosomes.

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