Electrophoretic isolation of extrachromosomal DNA from tumor cells
β Scribed by Donald R. Vandevanter; John C.-H. Tseng; Getachew Yirdaw
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 1013 KB
- Volume
- 12
- Category
- Article
- ISSN
- 1045-2257
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract Extrachromosomal circular DNAs ranging in size from submicroscopic molecules of approximately 100 kb to cytogenetically resolvable structures of 1000+ kb called minute and doubleβminute chromosomes have been shown to harbor amplified genes in primary tumor cells, tumor cell lines, and d
By cotransfecting plasmids carrying particular mutations in the beta-glucuronidase (GUS) gene into Nicotiana plumbaginifolia protoplasts and by monitoring the recombination rates using a recently developed transient assay, we were able to obtain insights into the mechanism of extrachromosomal recomb
A rapid method for the isolation of DNA from mouse cells grown in tissue culture is described. Cells are lysed in 0.24 M sodium phosphate, pH 6.8 buffer, containing 1% sodium dodecyl sulfate, 8 M urea, and 10m3 M ethylenediaminetetraacetic acid, and the crude lysate applied to a column of hydroxyapa