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Electrophoretic characterization of pyrimidine 5'-nucleotidase of human erythrocytes and its distinction from acid phosphatase

✍ Scribed by Raymonde Rosa; Colette Valentin; Jean Rosa

Book ID
115821929
Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
774 KB
Volume
79
Category
Article
ISSN
0009-8981

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πŸ“œ SIMILAR VOLUMES

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✍ Raymonde Rosa; Henri Rochant; Bernard Dreyfus; Colette Valentin; Jean Rosa πŸ“‚ Article πŸ“… 1977 πŸ› Springer 🌐 English βš– 418 KB

A new case of a defect in red cell pyrimidine 5'-nucleotide (P5N) activity was found in a large family from Guadeloupe in the West Indies. The propositus presented a characteristic hemolytic anemia with red cell basophilic stippling, an increased GSH level, and a shift of the peak in absorbance of n

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The mutant enzyme of a patient with hereditary pyrimidine 5'-nucleotidase deficiency was analyzed biochemically. Partially purified by DEAE-Sephadex and concentrated by ultrafiltration, the enzyme had a high Km for the substrate uridine monophosphate. Utilization of the substrate cytidine monophosph