Electrophoretic and chromatographic characterization of sulphur-rich proteins from wool

✍ Scribed by R.L. Darskus

• Book ID: 104144230
• Publisher: Elsevier Science
• Year: 1972
• Tongue: English
• Weight: 811 KB
• Volume: 69
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)92903-0

✦ Synopsis

The heterogeneity of the high-sulphur fraction of reduced and S-carbdxymethylated proteins from wool was studied by starch-gel electrophoresis in aqueous urea-acetic acid at pH 2.4. This system gives better resolution than previously used alkaline buffers. A typical preparation was found to give twelve major protein zones. A similar degree of resolution was obtained by chromatography on cellulose phosphate in aqueous urea-citrate buffer at pH 2.6 By chromatography of high-sulphur proteins on DEAR-cellulose at pH 4.5 and electrophoresis of the fractions at pH 2.4, a minimum of about thirty-six components could be identified.