With the demonstration of pronucleating and antinucleating proteins, the role of biliary proteins became of considerable research interest. Anionic polypeptide fraction is the third most abundant biliary protein; it is found in association with biliary lipids, has antinucleating properties for calci
Electrophoretic analysis of bile proteins from patients with and without gallstones
β Scribed by Yukiko Ota; Kenichi Ido; Ken Kimura; Emiko Fukui; Shigenori Ikemoto
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 460 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0173-0835
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The concentration of 25 major, minor and trace elements in human bile was determined by inductively coupled plasma atomic emission spectrometry. Gallbladder bile was obtained during surgery from patients with cholesterol gallstones, pigment stones and with no biliary tract abnormalities (controls).
Many studies have demonstrated that gallbladder bile (but not hepatic bile) of animals or patients with cholesterol gallstones contains higher protein concentrations than does gallbladder bile of control patients without stones or with pigment stones. The underlying defect has not been elucidated. T
The method for extracting Triton X-100 used by I. H. Mather and C. B. Tampling [Anal. B&hem. 93, 139-142 (1979)], has been extended to other detergents of dif%erent charge and chemical nature. All the detergents tested can be extracted with isopentanol in conditions in which not more than 8% of hydr