✦ LIBER ✦

Electrophoresis of the nucleic acids

✍ Scribed by Baldomero M. Olivera; Peter Baine; Norman Davidson

Publisher: Wiley (John Wiley & Sons)
Year: 1964
Tongue: English
Weight: 672 KB
Volume: 2
Category: Article
ISSN: 0006-3525
DOI: 10.1002/bip.1964.360020306

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✦ Synopsis

A zone electrophoresis apparatus using ultraviolet optics has been constructed to study nucleic acids at concentration leas than 0.004%. Native DNA has a mobility about 15% higher than denatured DNA over a range of conditions of p H and ionic strength.

A study of a molecular weight series of DNA indicates that the mobility is constant in the molecular weight range of 2 . 5 X 106 to 1.3 X 108. DNA mobilities change in the expected way with pH but the fractional change in mobility is less than the change in charge calculated by titration curves. A small decrease in mobility accompanies an increase in ionic strength. RNA's from various sources have mobilities slightly lower than denatured DNA except for s-RNA which travels slightly faster.

DNA's from different sources have closely similar mobilities.

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