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Electrophoresis of DNA. III. The effect of several univalent electrolytes on the mobility of DNA

✍ Scribed by Philip D. Ross; Robert L. Scruggs

Publisher
Wiley (John Wiley & Sons)
Year
1964
Tongue
English
Weight
339 KB
Volume
2
Category
Article
ISSN
0006-3525

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✦ Synopsis

The electrophoretic mobility of DNA in the presence of tetramethylammonium and alkali metal ion chlorides has been studied as a function of ionic strength. Each cation exhibits a characteristic behavior in accord with the idea that the order of interaction with DNA is Li+ > Na+ > K + > TMA+. The application of theories of the electrophoresis of polyelectrolytes is discussed, leading to an attempt to calculate the fractional charge per DNA phosphorus from the mobility data. Over the range 0.05-0.4M a constant and unique value of the DNA charge is obtained in the presence of each cation. Values of the zeta potential and of the friction factor per monomer unit are also ralrulated.

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