Electrophoresis of acidic glycosaminoglycans in hydrochloric acid: A micro method for sulfate determination

✍ Scribed by Erland Wessler

• Publisher: Elsevier Science
• Year: 1971
• Tongue: English
• Weight: 177 KB
• Volume: 41
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(71)90192-8

✦ Synopsis

Acidic glycosaminoglycans may by divided into several groups according to the structure of their polysaccharide backbone (1). Each group is heterogeneous with respect to molecular weight and, in most cases, with respect to charge density. The charge polydispersity reflects variations in the degree of sulfation. Current methods for determination of sulfate in acidic glycosaminoglycans arc laborious and require relatively large amounts of material. The present communication describes a simple micro method for estimating the sulfate content of glycosaminoglycans.