𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Electrophoresis of acid phosphohydrolase isozymes on cellogel

✍ Scribed by M.E. Hodes; M. Crisp; E. Gelb

Book ID
102984066
Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
680 KB
Volume
80
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

Methods are presented for the electrophoresis and detection of various acid phosphohydrolases on Cellogel. Isozymes of acid DNase, RNase, 3'phosphodiesterase, and nonspecific phosphodiesterase are readily detectable with these techniques. The enzymes studied were extracted from vertebrate spleens and leukocytes.

πŸ“œ SIMILAR VOLUMES

Two-dimensional electrophoresis of yeast
Two-dimensional electrophoresis of yeast histones on cellogel strips and polyacrylamide gels
✍ Martin Veth; Johann Sonnenbichler πŸ“‚ Article πŸ“… 1986 πŸ› Elsevier Science 🌐 English βš– 522 KB

For a quick analytical identification of histones a two-dimensional electrophoretic system has been developed. First the proteins are separated on cellulose acetate strips in alkaline buffer. Then they are reelectrophoresed in a second dimension on polyacrylamide gels either with sodium dodecyl sulf