✦ LIBER ✦

Electrophoresis and immunoblot of cerebrospinal fluid proteins in spasmodic torticollis

✍ Scribed by Dr. Paul A. Cullis; Laurace Townsend; Peter Lewitt; Nunzio Pomara; Darlene Reitz

Publisher: John Wiley and Sons
Year: 1986
Tongue: English
Weight: 704 KB
Volume: 1
Category: Article
ISSN: 0885-3185
DOI: 10.1002/mds.870010303

No coin nor oath required. For personal study only.

✦ Synopsis

Protein patterns of cerebrospinal fluid (CSF) from patients with spasmodic torticollis (ST) were investigated to determine whether abnormalities previously reported could be detected and further identified. CSF was collected from 12 patients with ST and 6 normal controls. The CSF proteins were analyzed using sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis and silver staining. In 1 1 of the 12 patients with ST, a CSF protein pattern was observed which differed from that in the controls. The identity of the abnormal proteins was ascertained by blotting and immunostaining with specific antisera to IgG and ceruloplasmin (Cp). CSF from 2 of 12 patients had distinct bands staining for IgG and 7 had abnormal immunostaining for Cp.

📜 SIMILAR VOLUMES

Cerebrospinal fluid γ-aminobutyric acid

Cerebrospinal fluid γ-aminobutyric acid in spasmodic torticollis

✍ R. J. Abbott; S. Currie; J. G. Howe; D. W. Sumner 📂 Article 📅 1983 🏛 John Wiley and Sons 🌐 English ⚖ 117 KB 👁 1 views

Paper electrophoresis of cerebrospinal f

Paper electrophoresis of cerebrospinal fluid proteins in patients with meningeal leukemia

✍ Arthur R. Rothman; Paul P. Carbone; Richard Rieselbach; Emil J Freireich 📂 Article 📅 1964 🏛 John Wiley and Sons 🌐 English ⚖ 358 KB 👁 2 views

## HE CLINICAL SYNDROME, MENINGEAL LEU- T kemia, is being increasingly recognized.11 Diagnosis presently depends on the identification of leukemic cells in the cerebrospinal fluid (CSF). In patients with meningeal leukemia, elevation of CSF protein concentration occurs inconsistently. The present

Comparison of oligoclonal immunoglobulin

Comparison of oligoclonal immunoglobulin G bands in multiple sclerosis cerebrospinal fluid by immunoblotting and immunofixation

✍ Pankaj D. Mehta; Bruce A. Patrick; Jeff Black 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 513 KB

## Comparison of oligoclonal immunoglobulin G bands in multiple sclerosis cerebrospinal fluid by immunoblotting and immunofixation We previously analyzed unconcentrated cerebrospinal fluid (CSF) from patients with multiple sclerosis (MS) and other neurologic diseases by isoelectric focusing in aga

Identification of proteins from human ce

Identification of proteins from human cerebrospinal fluid, separated by two-dimensional polyacrylamide gel electrophoresis

✍ Albert Sickmann; Wilma Dormeyer; Stefanie Wortelkamp; Dirk Woitalla; Wilfried Ku 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 294 KB 👁 2 views

The aim of this work is to display the protein composition of the cerebrospinal fluid by two-dimensional (2-D) gel electrophoresis and identify it using different mass spectrometric techniques. This will enable us to present an overview of the proteins in human cerebrospinal fluid. The comparison of

Horizontal two-dimensional electrophores

Horizontal two-dimensional electrophoresis of cerebrospinal fluid proteins with immobilized pH gradients in the first dimension

✍ Dr. Kari Mattila; Tuula Pirttilä; Harry Frey 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 257 KB 👁 2 views

The Use of High-Performance Electrophore

The Use of High-Performance Electrophoresis Chromatography for the Micropurification of Cerebrospinal Fluid Proteins in the Rat

✍ L. Saso; B. Silvestrini; C.Y. Cheng 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 803 KB

Using reversed-phase high-performance liquid chromatography (HPLC) on a Vydac C8 column in conjunction with sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) and silver staining, we have identified more than 30 proteins in cerebrospinal fluid collected from adult rats by cannula