A thin-layer cl~ron~atogral~hic technique using basic alumina layers and cleveloI~ment with acetone-water (S5 :x5 or 9 :I) has been clevised for the discrete separation of salts of compounds containing a single quatcrnary ammonium group, Quaternary ammonium cations were separated on basic alumina la
Electrophoresis and detection of proteins on thin layers of alumina
β Scribed by Dolores V. Lopiekes; Frank R. Dastoli; Steven Price
- Publisher
- Elsevier Science
- Year
- 1966
- Tongue
- English
- Weight
- 213 KB
- Volume
- 23
- Category
- Article
- ISSN
- 1873-3778
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β¦ Synopsis
24O in a glass chamber equilibrated at least 8 h previously with the solvent. The '1 plates were then dried and the spots located by spraying with concentrat,ed sulfuric acid containing 0.5 o/0 potassium dichromate. The spots were identified by use 'of reference standards. The respective findings are presented in Table I. Generally sharp separations resulted, 'except for mixtures, I and VIII.
Of great interest is the observation that the sequence of separation of components appeared to parallel the re-spective boiling points of the alcohols.
π SIMILAR VOLUMES
A sensitive immunochemical method was developed for the detection of glycosphingolipids on thin-layer chromatograms. The procedure involves oxidation of diol groups of glycosphingolipids with sodium periodate, derivatization of the formed aldehyde groups with digoxigenin-hydrazide, and reaction of t