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Electron microscopy of beaded agarose gels

✍ Scribed by T. K. Attwood; B. J. Nelmes; D. B. Sellen

Publisher
Wiley (John Wiley & Sons)
Year
1988
Tongue
English
Weight
698 KB
Volume
27
Category
Article
ISSN
0006-3525

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✦ Synopsis

Electron microscopy has been carried out on sections of beaded agarose with a wide range of thicknesses, and the results have been analyzed by means of stereological theory using computer graphics. The results agree with a randomly orientated system in which, for 4% gels, the mean molecular weight per unit length of the fiber system is 110 kg mol-' nm-', and the number average interjunction length is 37 nm, with an asymmetrical distribution resembling a Maxwell-Boltzmann distribution. The spatial distribution of the structure is not uniformly random and there seem to be microvoids.

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