A New Screen for Combinatorial Catalysis; On-Bead Testing in Agarose Gel

✍ Scribed by Michael Müller; Trevor W. Mathers; Anthony P. Davis

• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 118 KB
• Volume: 40
• Category: Article
• ISSN: 0044-8249
• DOI: 10.1002/1521-3773(20011015)40:20<3813::aid-anie3813>3.0.co;2-r

✦ Synopsis

Catalytic beads are readily distinguished from inactive controls in a method which exploits the localizing potential of a gel. A useful variant employs an acid/base indicator to detect the pH change on ester hydrolysis. Precipitation of the acidified indicator (red) creates an especially strong contrast between active and inactive beads.