Electrofusion for the pronuclear transplantation of mouse eggs
โ Scribed by Tsunoda, Y. ;Kato, Y. ;Shioda, Y.
- Book ID
- 111663239
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1987
- Weight
- 393 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0148-7280
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Pronuclear mouse eggs were maintained in globulin-citrate-Locke's solution for 120 to 180 minutes at one of the following temperatures: 22-23ยฐC (room), 35-36ยฐC (incubator) or 2-3ยฐC (refrigerator). Their viability was then compared with that of untouched eggs and with eggs immediately transferred fro
## Abstract Timing of development of naturally ovulated mouse eggs from sperm penetration to first cleavage, including that of DNA synthesis, was established. In an attempt to limit variability, partial synchronization of ovulation was accomplished by shortening the length of the dark period to fiv
A new and reliable pronuclear transplantation procedure for the mouse egg has been developed by McGrath and Solter ('83). To overcome the technical difficulties of such a procedure, especially in uniformly preparing enucleation pipettes and in reducing damages during micromanipulation, we have exami