Survival of pronuclear mouse eggs kept at different temperatures

✍ Scribed by Lin, T. P.

• Book ID: 102891120
• Publisher: John Wiley and Sons
• Year: 1968
• Tongue: English
• Weight: 756 KB
• Volume: 168
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/jez.1401680410

✦ Synopsis

Pronuclear mouse eggs were maintained in globulin-citrate-Locke's solution for 120 to 180 minutes at one of the following temperatures: 22-23°C (room), 35-36°C (incubator) or 2-3°C (refrigerator). Their viability was then compared with that of untouched eggs and with eggs immediately transferred from donor to host. After maintenance at room temperature the vitelline membrane shrank irregularly within the zona pellucida; on the other hand, in the pronuclear eggs maintained at refrigeration temperature this did not occur. In contrast, the cytoplasm of the incubated eggs was highly condensed and the vitellus shrunken, although the vitelline surface remained smooth. Survival rate of eggs, as tested by transplantation, was lower after incubation than after refrigeration. Body weight of fetuses developed from eggs maintained at room temperature and refrigeration temperature was significantly less than that of fetuses developed from untouched control eggs, from eggs immediately transferred to a host animal, or from eggs maintained at incubation temperature.