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Electroelution of nucleic acids from polyacrylamide gels: A custom-made, agarose-based electroeluter

โœ Scribed by Fadouloglou, Vasiliki E.

Book ID
122452615
Publisher
Elsevier Science
Year
2013
Tongue
English
Weight
331 KB
Volume
437
Category
Article
ISSN
0003-2697

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Rapid electroelution of nucleic acids fr
Rapid electroelution of nucleic acids from agarose and acrylamide gels
โœ H.Peter Zassenhaus; Ronald A. Butow; Yolanda P. Hannon ๐Ÿ“‚ Article ๐Ÿ“… 1982 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 880 KB

We describe a procedure for the electroelution of nucleic acids from agarose or acrylamide gels. The method employs a combination of low-and high-ionic-strength buffers so that nucleic acids electroelute under a high-voltage gradient but accumulate over a high-salt barrier. By this means electroelut