Electrode potential-controlled DNA damage in the presence of copper ions and their complexes

✍ Scribed by Miroslav Fojta; Ludĕk Havran; Tatiana Kubičárová; Emil Paleček

• Publisher: Elsevier Science
• Year: 2002
• Tongue: English
• Weight: 79 KB
• Volume: 55
• Category: Article
• ISSN: 1567-5394
• DOI: 10.1016/s1567-5394(01)00131-1

✦ Synopsis

Supercoiled (sc) DNA immobilized at the surface of a hanging mercury drop electrode was cleaved by reactive oxygen species generated by an electrochemically modulated reaction of copper ions, hydrogen peroxide and/or oxygen. The cleavage was observed in a certain potential region where redox cycling of DNA-bound Cu(II)/Cu(I) took place. In the presence of 1,10-phenanthroline the maximum efficiency of DNA cleavage was shifted to more negative potentials and the effect was enhanced.