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Electrochemical Studies of Vitamin K3 Interacting with Hemoglobin and Determination of Hemoglobin

✍ Scribed by Zhiwei Zhu; Nan-Qiang Li

Publisher: John Wiley and Sons
Year: 1999
Tongue: English
Weight: 128 KB
Volume: 11
Category: Article
ISSN: 1040-0397
DOI: 10.1002/(sici)1521-4109(199911)11:15<1145::aid-elan1145>3.0.co;2-8

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✦ Synopsis

Electrochemical investigation of the interaction of vitamin K 3 (VK 3 ) with hemoglobin (Hb) on a Hg electrode is reported for the ®rst time. With the addition of hemoglobin in vitamin K 3 solution, both the reduction and oxidation currents decrease but with no change of peak potentials. The reaction of vitamin K 3 with hemoglobin forms a kind of nonelectrochemical active supramolecular complex Hb±VK 3 . The equilibrium constant for complex is calculated as 3.75610 4 . A satisfactory result has been obtained for the determination of hemoglobin in clinical blood samples.

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