Interaction of trout hemoglobin with H2O2: a chemiluminescence study
✍ Scribed by Gabbianelli, Rosita ;Falcioni, Giancarlo ;Santroni, Anna Maria ;Fiorini, Rosamaria ;Coppa, Giovanni Valentino ;Kantar, Ahmad
- Publisher
- John Wiley and Sons
- Year
- 1997
- Weight
- 115 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0884-3996
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✦ Synopsis
Erythrocytes from trout Salmo irideus are characterized by four different hemoglobin components (HbI, HbII, HbIII and HbIV), HbI and HbIV being predominant. In this study we describe the interaction between trout hemoglobin (HbI and HbIV) and H 2 O 2 using a chemiluminescence assay. Our data show that the reaction of hemoglobins with H 2 O 2 produces a time-limited and significant increase of chemiluminescence signal. The half-life of the decay of this chemiluminescence signal was characteristic for each type of hemoglobin used. These results indicate the formation of excited molecules related to the interaction between trout hemoglobin and H 2 O 2 .
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Visible oscillating chemiluminescence (CL) of luminol-H2O2-KSCN-CuSO4 was studied using the organic base (2-hydroxyethyl)trimethylammonium hydroxide. The effect of concentrations of luminol, H2O2, KSCN, CuSO4 and the base were investigated in a batch reactor. This report shows how the concentration