Electrochemical Studies of Intramolecular Electron Transfer in Laccase from Trametes versicolor

✍ Scribed by Dmitri Ivnitski; Plamen Atanassov

• Book ID: 102830886
• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 521 KB
• Volume: 19
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/elan.200703983

✦ Synopsis

Abstract

This paper describes electrochemical behavior of laccase from the fungus Trametes versicolor. The issues related to discrimination of the redox potentials corresponding to copper centers T1 and T2/T3 in the active site and possible mechanism of intramolecular electron transfer have been discussed. The electron‐transfer rate constant for laccase immobilized on carbon electrode is 3.4 s^−1^. The bioelectrocatalytic activity of the enzyme was studied in the presence of 1,4‐hydroquinone (HQ). The kinetics of HQ oxidation is very fast (K~M~=3.8 μM). However, the catalytic activity of laccase in the presence of high concentration of HQ decreases drastically. It is suggested that the T2/T3 copper center is able to accept electrons from HQ molecules directly via intramolecular channel.