Electrochemical detection of proteins in high-performance liquid chromatography using on-line, postcolumn photolysis

✍ Scribed by Lin Dou; Anders Holmberg; Ira S. Krull

• Publisher: Elsevier Science
• Year: 1991
• Tongue: English
• Weight: 811 KB
• Volume: 197
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(91)90407-k

✦ Synopsis

Direct detection of proteins in high-performance liquid chromatography electrochemistry (LCEC) is difficult. By using on-line, postcolumn photolysis, proteins now can be detected by LCEC at microgram per milliliter levels. The compatibilities of size exclusion chromatography (SEC), reversed-phase chromatography (RPC), ion-exchange chromatography (IEC), and hydrophobic interaction chromatography (HIC) with photolysis-electrochemical detection is described for proteins together with the analytical figures of merit. Inherent from the advantages of electrochemical detection, the method is sensitive and selective.