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Electrochemical Detection of Nitric Oxide in Biological Systems

✍ Scribed by Andrew J. Gow; Stephen R. Thom; Clifford Brass; Harry Ischiropoulos

Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
96 KB
Volume
56
Category
Article
ISSN
0026-265X

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✦ Synopsis

Nitric oxide is a key regulator of diverse physiological processes that include vasoregulation, platelet and neutrophil adherence, and immune defense. The detection and quantification of nitric oxide has been accomplishd by a variety of methodologies. In this paper we desribe methodologies to detect nitric oxide in solution using an electrochemical detector and provide examples of nitric oxide detection in biological systems including data from plasma and cell suspensions. The experiments outlined show that electrochemical detection is suitable for detecting and quantifying nitric oxide within biological samples and buffers. Potential pitfalls and necessary controls are discussed. The use of electrochemical detection was also found to be useful in determining the flux of nitric oxide produced by donor species in different biological buffers.

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