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Electrochemical Cholesterol Sensor Based on Tin Oxide-Chitosan Nanobiocomposite Film

✍ Scribed by Anees A. Ansari; Ajeet Kaushik; Pratima R. Solanki; B. D. Malhotra

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
482 KB
Volume
21
Category
Article
ISSN
1040-0397

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✦ Synopsis

Abstract

A chitosan (CS)‐tin oxide (SnO~2~) nanobiocomposite film has been deposited onto an indium‐tin‐oxide glass plate to immobilize cholesterol oxidase (ChOx) for cholesterol detection. The value of the Michaelis–Menten constant (K~m~) obtained as 3.8 mM for ChOx/CS‐SnO~2~/ITO is lower (8 mM) than that of a ChOx/CS/ITO bioelectrode revealing enhancement in affinity and/or activity of ChOx towards cholesterol and also revealing strong binding of ChOx onto CS‐SnO~2~/ITO electrode. This ChOx/CS‐SnO~2~/ITO cholesterol sensor retains 95% of enzyme activity after 4–6 weeks at 4 °C with response time of 5 s, sensitivity of 34.7 μA/mg dL^−1^ cm^2^ and detection limit of 5 mg/dL.

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