Electrochemical characteristics of a carbon-based thick-film l-lactate biosensor using l-lactate dehydrogenase

✍ Scribed by Hyun C Yoon; Hak-Sung Kim

• Publisher: Elsevier Science
• Year: 1996
• Tongue: English
• Weight: 748 KB
• Volume: 336
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/s0003-2670(96)00445-x

✦ Synopsis

Carbon-based thick-film electrodes employing nicotinamide adenine dinucleotide (NAD')-dependent dehydrogenases were constructed by screen printing. Cyclic voltammetric and amperometric investigations of the thick-film electrode showed significantly different electrocatalytic properties toward NADH compared to conventional polished electrodes. Electrochemical oxidation of NADH was attained at the low potential of +350 mV vs. Ag/AgCl at the thick-film carbon electrode, and this can be attributed to increased electrocatalytic sites due to the rough and jagged electrode surface and the formation of a microelectrode array-like structure at the electrode surface. Based on these observations, a thick-film lactate biosensor using lactate dehydrogenase was developed. Analytical performance of the resulting lactate biosensor was evaluated in terms of sensitivity and stability.