A microdialysis fibre based sampler for flow injection analysis: determination of L-lactate in biofluids by an electrochemically synthesised bilayer membrane based biosensor

✍ Scribed by F. Palmisano; D. Centonze; M. Quinto; P.G. Zambonin

• Publisher: Elsevier Science
• Year: 1996
• Tongue: English
• Weight: 506 KB
• Volume: 11
• Category: Article
• ISSN: 0956-5663
• DOI: 10.1016/0956-5663(96)82737-0

✦ Synopsis

A microdialysis fibre based, low volume sampler is described which can be used in flow injection analysis (FIA) when an on-line dilution of the sample and/or removal of high molecular weight interferents is required. This device used in combination with a lactate amperometric biosensor based on lactate oxidase electrochemically immobilised in a bilayer membrane of poly(o-phenylendiamine) and overoxidized poly(pyrrole) permits the extension of the linear range of response up to 10 mM lactate. Combining microdialysis sampling with FIA and amperometric detection at an interference-free and fast-response biosensor, lactate determination in complex media such as serum, milk and yoghurt can be easily achieved with a high sample throughput and no sample pre-treatment.