Electroanalytical Determination of Tungsten and Molybdenum in Proteins

✍ Scribed by Peter L. Hagedoorn; Petra van't Slot; Herman P. van Leeuwen; Wilfred R. Hagen

• Publisher: Elsevier Science
• Year: 2001
• Tongue: English
• Weight: 112 KB
• Volume: 297
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.2001.5300

✦ Synopsis

Recent crystal structure determinations accelerated the progress in the biochemistry of tungsten-containing enzymes. In order to characterize these enzymes, a sensitive determination of this metal in protein-containing samples is necessary. An electroanalytical tungsten determination has successfully been adapted to determine the tungsten and molybdenum content in enzymes. The tungsten and molybdenum content can be measured simultaneously from 1 to 10 g of purified protein with little or no sample handling. More crude protein samples require precipitation of interfering surface active material with 10% perchloric acid. This method affords the isolation of novel molybdenumand tungsten-containing proteins via molybdenum and tungsten monitoring of column fractions, without using radioactive isotopes. A screening of soluble proteins from Pyrococcus furiosus for tungsten, using anion-exchange column chromatography to separate the proteins, has been performed. The three known tungsten-containing enzymes from P. furiosus were recovered with this screening.