Electric-field-induced fusion of evacuolated mesophyll protoplasts of oat
β Scribed by R. Hampp; M. Steingraber; W. Mehrle; U. Zimmermann
- Publisher
- Springer
- Year
- 1985
- Tongue
- English
- Weight
- 505 KB
- Volume
- 72
- Category
- Article
- ISSN
- 0028-1042
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π SIMILAR VOLUMES
Under the action of a high electric field pulse a pronounced stimulation of yeast protoplast fusion initiated by polyethylene glycol and Ca (2+) ions was found. The fusion rate was enhanced by a factor of 200 for different genetically marked strains of the yeast Saccharomyces cerevisiae as compared
## Abstract Prototrophic colonies could be selected on minimal medium after mixing of protoplasts from diauxotrophic mutants of the yeasts __Saccharomycopsis lipolytica__ and/or __Lodderomyces elongisporus__ and treatment with polyethylene glycol (PEG) in the presence of calcium chloride. This is t