Efficient removal of LoxP-flanked genes by electroporation of Cre-recombinase mRNA
β Scribed by Dave Van den Plas; Peter Ponsaerts; Viggo Van Tendeloo; Dirk R Van Bockstaele; Zwi N Berneman; Joseph Merregaert
- Book ID
- 117053818
- Publisher
- Elsevier Science
- Year
- 2003
- Tongue
- English
- Weight
- 335 KB
- Volume
- 305
- Category
- Article
- ISSN
- 0006-291X
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## Abstract Summary: Conditional and tissue specific gene targeting using the CreβloxP recombination system in combination with established ES cell techniques has become a standard for in vivo loss of function studies. In a typical flox and delete gene targeting strategy, the loxPβneoβloxP cassette