𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Efficient plant regeneration from rice protoplasts in general medium

✍ Scribed by Zhijian Li; Norimoto Murai

Publisher
Springer
Year
1990
Tongue
English
Weight
977 KB
Volume
9
Category
Article
ISSN
0721-7714

No coin nor oath required. For personal study only.

✦ Synopsis

We established an efficient and reproducible procedure for protoplast propagation and fertile plant regeneration of rice (Oryza sativa L.) cultivars Nipponbare and Taipei 309. Selection of scutellum-derived secondary calli, the use of General medium and nurse culture were all found to be critical in the procedure. When 5 basal media (Murashige and Skoog, RY-2, modified R2, Amino Acid and General media) were compared, suspension callus growth rate, protoplast yield and plating efficiency were all about 30% higher in General medium than in the second-best R2 medium. Only one month was required to develop suspension cultures for protoplast isolation using General medium. A plating efficiency as high as 17% and a plant regeneration frequency of 67% were achieved by the improved procedure. Agronomic traits of protoplast- and seed-derived plants were found to be similar.

πŸ“œ SIMILAR VOLUMES

Plant regeneration from protoplasts of i
Plant regeneration from protoplasts of indica rice: genotypic differences in culture response
✍ J. Kyozuka; E. Otoo; K. Shimamoto πŸ“‚ Article πŸ“… 1988 πŸ› Springer 🌐 English βš– 698 KB

Fourteen varieties of indica rice (Oryza saliva L.) were examined for their capacity for plant regeneration from protoplasts using the nurse culture methods developed for japonica rice. Calli induced from germinating seeds were grouped into two types: type I, white and compact; type II, yellow and f

Rapid and efficient plant regeneration f
Rapid and efficient plant regeneration from hypocotyl protoplasts ofBrassica carinata
✍ S. B. Narasimhulu; P. B. Kirti; S. Prakash; V. L. Chopra πŸ“‚ Article πŸ“… 1992 πŸ› Springer 🌐 English βš– 345 KB

Protoplasts were isolated from hypocotyls of 7-d-old seedlings of three genotypes of Brassica carinata after enzymatic digestion in cellulase R-10 (0.5%) and pectolyase Y-23 (0.025%). The protoplasts were stabilized with 0.4 M mannitol used as osmoticum, and were cultured in darkness in Kao's liquid

An improved procedure for plant regenera
An improved procedure for plant regeneration from indica and japonica rice protoplasts
✍ R. K. Jain; G. S. Khehra; S. -H. Lee; N. W. Blackball; R. Marchant; M. R. Davey; πŸ“‚ Article πŸ“… 1995 πŸ› Springer 🌐 English βš– 529 KB

Plant regeneration from protoplasts of two commercially cultivated Indian indica rice varieties, Pusa Basmati 1 and Java, has been accomplished by plating embryogenic cell suspension-derived protoplasts on the surface of filter membranes overlying agarose-embedded feeder cells of Lolium multltifloru