Efficient gene transfer to human squamous cell carcinomas by the herpes simplex virus type 1 amplicon vector
β Scribed by John F. Carew; Howard Federoff; Marc Halterman; Dennis H. Kraus; Howard Savage; Peter G. Sacks; Stimson P. Schantz; Jatin P. Shah; Yuman Fong
- Book ID
- 117026811
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 338 KB
- Volume
- 176
- Category
- Article
- ISSN
- 0002-9610
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We have analyzed the ability of a recombinant replication-defective adenovirus to transfer the thymidine kinase gene of herpes simplex virus (HSV-tk) into hepatocellular carcinoma (HCC) cells to confer sensitivity to ganciclovir. Three HCC cell lines (Hep3B, PLC/PRF/5, and HepG2) were efficiently in
## Abstract To analyze the site of integration of the herpes simplex virus type 1 (HSVβ1) thymidine kinase (TK) gene in biochemically transformed human cells, TKβHeLaβ(BU25) cells were transformed to the TK^+^ phenotype by a cloned, 2 kbp Pvull fragment of HSVβ1 DNA. The transformed cells [HeLa(BU2