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Efficient expression of foreign genes in CHO DHFR− cellsby electroporation

✍ Scribed by Wen-Zhen Lin; Susanna S.T. Lee; Wing-Tai Cheung

Book ID
113491737
Publisher
Elsevier Science
Year
2009
Tongue
English
Weight
180 KB
Volume
37
Category
Article
ISSN
1045-1056

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We previously reported the expression of human beta-interferon (B-IFN) (Miyaji et al., 1989) and human lymphotoxin (Miyaji et al., 1990) in Namalwa KJM-1 cells adapted to serum-free medium. To establish an efficient gene expression system, a dihydrofolate reductase (dhfr) gene coamplification method