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Efficient Estimation of Protein Binding Constants

✍ Scribed by J. Connett; A. Gharrett; R. McHugh

Publisher
John Wiley and Sons
Year
1985
Tongue
English
Weight
282 KB
Volume
27
Category
Article
ISSN
0323-3847

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✦ Synopsis

Assays of cyclic A L W typically employ a Scatchard-like data analysis to estimate the total binding protein RT and the dissociation constant K& The linear regression procedure employed is statistically ad hoc because the response variable (bound counta) occurs on both sides of the Scatchard equation.

The alternative analysis presented here is derived fro,m the cyclic AMP binding reaction. The resulting data analysis, employing non-linear least squares, resulta in estimators that 011 the basis of Monte Carlo studies have less bias and greater precision than the Scatchard method.

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