✦ LIBER ✦

Estimation of protein-ligand binding parameters from continuous ultrafiltration results

✍ Scribed by John A. Steele; Richard D. Durbin

Publisher: Elsevier Science
Year: 1976
Weight: 281 KB
Volume: 6
Category: Article
ISSN: 0010-468X
DOI: 10.1016/0010-468x(76)90039-8

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✦ Synopsis

A method of analyzing the result of continuous ultrafiltration experiments to obtain protein-ligand binding parameters is presented. This method employs a nonlinear least-squares regression algorithm coupled with a model of protein-ligand binding which alloww the computation of free ligand concentrations, and a second-order Runge-Kutta method to integrate free concentrations with respect to collected ultrafiltrate. The approach is general and effectively removes the constraints on maximum fraction size imposed by other methods.

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