Effects of valinomycin on hexose transport and cellular ATP pools in mouse fibroblasts

✍ Scribed by Kiyofumi Yamanishi

• Publisher: John Wiley and Sons
• Year: 1984
• Tongue: English
• Weight: 789 KB
• Volume: 119
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041190204

✦ Synopsis

The K + ionophore valinomycin at concentrations of 1 x lo-' M and over, stimulated 2-deoxy-D-glucose (2DG) and 3-0-methylglucose (30MG) uptake in Swiss 3T3 fibroblasts. The rate-limiting step of 2DG uptake was transport rather than phosphorylation, in the control or valinomycin-treated cells. Kinetic analysis showed that valinomycin increased the V, , , for 2DG uptake without change of the K, . The valinomycin-stimulated 2DG uptake was insensitive to 10 pglml cycloheximide, and extracellular K + concentrations between 0.1 and 50 mM. On the other hand, valinomycin at the concentration of 1 x water-saturated atmosphere containing 5% C02 in air. Three days later, dishes containing monolayer cells which had just become confluent were used for experiments.

Measurement of hexose uptake

The uptake of 2-deoxy-D-glucose (2DG) or 30MG by Swiss 3T3 cells was measured as described previously 3 x lo5 cells/2 ml or 1.5 x 10 ? cells/lO ml into plastic