Modulation of intracellular potassium and ATP: Effects on coated pit function in fibroblasts and hepatocytes
✍ Scribed by Janet M. Larkin; William C. Donzell; Richard G. W. Anderson
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- English
- Weight
- 999 KB
- Volume
- 124
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
✦ Synopsis
Previously we reported that cultured human fibroblasts depleted of intracellular potassium ( K + ) had a reduced number of surface coated pits and were unable to internalize receptor-bound molecules such as low density lipoprotein (LDL). We have extended these studies in two important ways. First, we have developed a method for modulating the number of coated pits in situ. Human fibroblasts incubated in K+-free buffer that contains 4pm nigericin rapidly become depleted of K + and lose the ability to internalize 1251-LDL. When rat livers are perfused with the same buffer, there is a 75% decrease in the number of surface coated pits in hepatocytes. Secondly, we have explored the possibility that K+-depletion effects coated pit function by lowering intracellular ATP. We found that although this protocol lowers intracellular