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Effects of Tryptophan Mutation on the Thermal Stability and Catalytic Activity of Cold-Adapted Esterase At Ambient Temperatures

โœ Scribed by Jang, Sei-Heon; Boyineni, Jerusha; Kim, Junyoung; Lee, ChangWoo


Book ID
122086768
Publisher
Biophysical Society
Year
2014
Tongue
English
Weight
58 KB
Volume
106
Category
Article
ISSN
0006-3495

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โœ Ljudmila Kulakova; Andrey Galkin; Toru Nakayama; Tokuzo Nishino; Nobuyoshi Esaki ๐Ÿ“‚ Article ๐Ÿ“… 2004 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 333 KB

The gene encoding an esterase (PsyEst) of Psychrobacter sp. Ant300, a psychrophilic bacterium isolated from Antarctic soil, was cloned, sequenced, and expressed in Escherichia coli. PsyEst, which is a member of hormone-sensitive lipase (HSL) group of the lipase/esterase family, is a cold-active, the