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Effects of the sample matrix on the separation of peptides by high performance capillary electrophoresis

✍ Scribed by Satow, Takashi ;Machida, Akiko ;Funakushi, Kayo ;Palrnieri, Richard

Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
442 KB
Volume
14
Category
Article
ISSN
0935-6304

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✦ Synopsis

Abstract

The effects of the sample matrix on the separation of peptides by HPCE has been investigated. Under both acidic and alkaline conditions, use of 25–30 mM salts in the sample zone resulted in much better resolution than did 100 mM salts. Prefocusing effects and acceleration of elution were also observed. These results agree well with the theory developed by Everaerts. In this paper a practical guide for high sensitivity, high resolution separation of salt‐containing peptide mixtures is proposed.

πŸ“œ SIMILAR VOLUMES

On the nature of the forces controlling
On the nature of the forces controlling selectivity in the high performance capillary electrochromatographic separation of peptides
✍ Karin Walhagen; Marion I. Huber; Tom P. Hennessy; Milton T. W. Hearn πŸ“‚ Article πŸ“… 2003 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 319 KB

## Abstract In this minireview, the nature of the forces controlling selectivity in the high performance capillary electrochromatographic (HP‐CEC) separation of peptides has been examined. For uncharged and charged peptides, a synergistic interplay occurs in HP‐CEC systems between adsorptive/partit