Abstract
Hydroxyapatite (HA)/collagen composites were reported to induce bony growth. Various methods for preparing HA‐based composites have been investigated as potential biomaterials for bone substitutes. However, no method can generate a thick nanoporous HA. A novel bone regenerative nanocomposite consisting of nano‐hydroxyapatite (HA), nano‐amorphous calcium phosphate (ACP) and reconstituted collagen by electrodeposition was designed in this research. Specimens with and without nanoporosity were evaluated using electrochemical measurements, material analyses, and cell‐material interactions. The results showed that reconstituted collagen/nano‐(HA and ACP) illustrated a multinanoporous structure and enhanced biocompatibility. Nanocomposite was comprised to nano‐(HA and ACP) and reconstituted collagen. The core cell structure was formed during electrodeposition. Nanoporosity and nanostructure were observed as formation of nanocomposite. The nano‐(HA and ACP) phases were essentially composed of a nanoporous and nanostructural biocomposite. Reconstituted collagen incorporation with the nanoporous and nanostructural biocomposite significantly facilitated the osteogenic differentiation of mesenchymal stem cells. Reconstituted collagen was covered with nano‐(HA and ACP), profoundly impacting the enhancement of biocompatibility on application of implant and tissue engineering. The bioactive nano‐HA/reconstituted collagen‐induced osteogenic differentiation of mesenchymal stem cells enables to enhance bone growth/repair and osseointegration. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010