𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Effects of the calcium influx inhibitor carboxyamido-triazole on the proliferation and invasiveness of human prostate tumor cell lines

✍ Scribed by William J. Wasilenko; Ann J. Palad; Kenneth D. Somers; Peter F. Blackmore; Elise C. Kohn; Johng S. Rhim; George L. Wright Jr.; Paul F. Schellhammer

Publisher
John Wiley and Sons
Year
1996
Tongue
French
Weight
658 KB
Volume
68
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Aberrant cellular signaling is a central feature of malignant cells and a potential target for anti-cancer therapy. Carboxyamido-triazole (CAI) is a calcium influx inhibitor that alters calcium-sensitive signal transduction pathways and suppresses the proliferative and metastatic potential of malignant cells. We have examined the effects of CAI on several tumor-associated parameters in human prostate cancer cell lines to evaluate the potential of CAI as a signal-transduction therapy agent for advanced-stage prostate cancer. Measuring anchorage-dependent cell growth, continuous application of CAI inhibited the growth of DU-145, PPC-1, PC3 and LNCaP tumor cells with 50% inhibitory concentrations ranging 10-30 microM. Direct cell enumeration assays revealed that the growth-suppressing activity of CAI toward DU-145 cells was reversible, indicating a cytostatic effect of the drug on tumor cells. The drug also inhibited the proliferation of several immortalized human prostatic epithelial cell lines. The proliferation of HaCaT- and RHEK-1-immortalized keratinocyte cell lines was relatively insensitive to CAI. Additionally, invasion by DU-145, PC3 and PPC-1 cells through Matrigel in vitro was reduced approximately 60-70% by 10 microM CAI. Other cellular effects of CAI included an attenuation of the elevation of intracellular free calcium in response to bombesin and carbachol in PC3 cells and a marked dose-dependent inhibition of prostate-specific antigen secretion in LNCaP cell cultures.

πŸ“œ SIMILAR VOLUMES

Effects of tyrosine kinase inhibitors on
Effects of tyrosine kinase inhibitors on the proliferation of human breast cancer cell lines and proteins important in the RAS signaling pathway
✍ Jeffrey W. Clark; Anabela Santos-Moore; Lisa E. Stevenson; A. Raymond Frackelton πŸ“‚ Article πŸ“… 1996 πŸ› John Wiley and Sons 🌐 French βš– 698 KB

Breast cancers frequently over-express a number of growth factor receptors. In addition, elevated src family kinase activity is present in a percentage of these neoplasms and has been implicated in signal transduction in these cells. Therefore, inhibiting tyrosine kinase activity is a potential appr

An overview of the effect of linoleic an
An overview of the effect of linoleic and conjugated-linoleic acids on the growth of several human tumor cell lines
✍ Marina Maggiora; Mauro Bologna; Maria Paola CerΓΉ; Laura Possati; Angelo Angelucc πŸ“‚ Article πŸ“… 2004 πŸ› John Wiley and Sons 🌐 French βš– 320 KB πŸ‘ 1 views

## Abstract Both n‐6 and n‐3 polyunsaturated fatty acids are dietary fats important for cell function, being involved in several physiologic and pathologic processes, such as tumorigenesis. Linoleic acid and conjugated linoleic acid, its geometrical and positional stereoisomer, were tested on sever

Effects of the tyrosine-kinase inhibitor
Effects of the tyrosine-kinase inhibitor geldanamycin on ligand-induced HER-2/NEU activation, receptor expression and proliferation of HER-2-positive malignant cell lines
✍ Frank Hartmann; Eva M. Horak; Cheryl Cho; Ruth Lupu; Joseph B. Bolen; Mary A. St πŸ“‚ Article πŸ“… 1997 πŸ› John Wiley and Sons 🌐 French βš– 408 KB πŸ‘ 2 views

Geldanamycin belongs to the family of benzoquinoid ansamycin tyrosine-kinase inhibitors. We have examined its effects on Her-2/neu kinase activity, protein expression level, and proliferation of Her-2+ malignant cells. In SK-BR-3 breast-cancer cells, short-time treatment with geldanamycin completely