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Effects of the antimicrotubular cancerostatic drug nocodazole on the yeast Saccharomyces cerevisiae

✍ Scribed by Dr. W. Künkel

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
792 KB
Volume
20
Category
Article
ISSN
0233-111X

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✦ Synopsis

Abstract

Nocodazole completely inhibited cell division of Saccharomyces cerevisiae, contrary to methylbenzimidazole‐2‐ylcarbamate, estimated by cell counting. Growth as measured by turbidity and dry weight estimations, however, was not influenced. Treatment for two hours with nocodazole interrupts the budding cycle of the yeast within a period when bud and mother cells have reached equal sizes. Dependent on duration of nocodazole treatment stretched or dumb‐bell shaped nuclei are localized between bud and mother cell or two daughter nuclei are present.

It has been shown by electron microscopy that nocodazole neither destroyed karyo‐ nor ground‐plasmic microtubules. The spindle pole bodies (spb), however, reached double the normal size approximately.

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