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Effects of supplementation of BH4 after prolonged ischemia in skeletal muscle

โœ Scribed by Wei Z. Wang; Xin-Hua Fang; Linda L. Stephenson; Kayvan T. Khiabani; William A. Zamboni

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
165 KB
Volume
27
Category
Article
ISSN
0738-1085

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โœฆ Synopsis

Abstract

Purpose: To determine whether the supplementation of tetrahydrobiopterin (BH~4~, an essential cofactor of nitric oxide synthase; NOS) could attenuate endothelial dysfunction and improve NOS activity and cell viability in skeletal muscle after ischemia/reperfusion (I/R). __Methods:__A vascular pedicle isolated rat cremaster muscle model was used. Cremaster muscles were subjected to 4 h of ischemia followed by 2 h of reperfusion. Rats were given either normal saline or BH~4~ by intravenous injection at 1 min prior to reperfusion. After reperfusion, average arteriole diameter, capillary perfusion, endothelialโ€dependent/โ€independent vasodilatation, NOS activity, and muscle cell viability were evaluated. Results: Supplementation of BH~4~ prior to reperfusion significantly attenuated reperfusionโ€induced vasoconstriction, poor capillary perfusion, and endothelial dysfunction and enhanced cNOS activity and slightly improved cell viability in the skeletal muscle after I/R. Conclusion: Supplementation of BH~4~ during reperfusion provided a significant protection against I/R injury in rat skeletal muscle. ยฉ 2007 Wileyโ€Liss, Inc. Microsurgery, 2007.

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