Characterization of microvascular vasoconstriction following ischemia/reperfusion in skeletal muscle using videomicroscopy

✍ Scribed by Mark Pemberton; Gary L. Anderson; John H. Barker

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 850 KB
• Volume: 17
• Category: Article
• ISSN: 0738-1085
• DOI: 10.1002/(sici)1098-2752(1996)17:1<9::aid-micr2>3.0.co;2-k

✦ Synopsis

This study investigated the possible contribution of microvascular vasoconstriction to no-reflow following ischemiakeperfusion in a mouse skeletal muscle model. Using paired cremaster muscles, arterioles of diameter 10-100 pm were directly viewed and measured by the use of an in vivo videomicroscopy before and after a 6-hr period of complete ischemia at 27°C. Following ischemiaheperfusion, feeder and arcading arterioles constricted significantly to 54.5 and 62% of pre-ischemic baseline diameters respectively ( P < .05). While the calcium antagonist diltiazem and nitroprusside were both able to reverse arteriolar constriction, endothelium-dependent acetylcholine-induced dilatation was markedly impaired following reperfusion (P < 0.05). Superoxide dismutase did not attenuate the microvascular response, suggesting that the mechanism is likely to be at least partly free radical-independent.