𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Effects of sulpiride on mRNA levels of steroid 5α-reductase isozymes in prostate of adult rats

✍ Scribed by Pilar Sánchez; Jesús M. Torres; Pablo Vílchez; Raimundo G. del Moral; Esperanza Ortega

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
117 KB
Volume
60
Category
Article
ISSN
1521-6543

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Prolactin (PRL) is implicated in prostate growth and in the development and regulation of benign prostatic hypertrophy (BPH) and prostate cancer (PCa). PRL may exert its effects on prostate in synergism with androgens. The most active androgen in the prostate is the 5α‐dihydrotestosterone (DHT) obtained from testosterone by the 5α‐reductase (5α‐R) enzyme, which is expressed in the prostate as two isozymes, 5α‐R1 and 5α‐R2. In this study, sulpiride, a prolactin‐secretion inductor, was administered to male rats. mRNA levels of 5α‐R1 and 5α‐R2 were measured in prostate of controls and sulpiride‐treated rats, using one‐step quantitative RT‐PCR coupled with laser‐induced fluorescence capillary electrophoresis (LIF‐CE). Results demonstrated that sulpiride‐induced hyperprolactinemia is associated with an increase in mRNA levels of both 5α‐R1 and 5α‐R2 in prostate of adult rats. Although a direct effect of sulpiride on prostate gland cannot be ruled out, hyperprolactinemia may be a factor to be considered in aging males, in whom prostatic diseases such as BPH and PCa are more frequent. © 2007 IUBMB IUBMB Life, 60(1): 68–72, 2008

📜 SIMILAR VOLUMES

Identification and partial characterizat
Identification and partial characterization of two steroid 5α-reductase isozymes in the canine prostate
✍ Span, Paul N.; Schalken, Jack A.; Sweep, Fred G.J.; Smals, Anthony G.H. 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 123 KB 👁 1 views

## BACKGROUND. The dog has been extensively used as an in vivo model to test the pharmacokinetics and effects on pathological prostatic growth of 5␣-reductase inhibitors. However, no information is available on the existence or characteristics of canine 5␣-reductase isozymes. METHODS. The 5␣-reduc

Effect of turosteride, a 5α-reductase in
Effect of turosteride, a 5α-reductase inhibitor, on the Dunning R3327 rat prostatic carcinoma
✍ Zaccheo, T.; Giudici, D.; di Salle, E. 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 351 KB 👁 1 views

BACKGROUND. Turosteride (FCE 26073) is a new, potent, and selective 5␣-reductase inhibitor. We have investigated its effect on tumor growth, organ weight, and serum hormone levels in rats bearing the androgen sensitive Dunning R3327 prostatic carcinoma. METHODS. Animals with tumor diameters of 0.5-1

Effect of early treatment of prostate ca
Effect of early treatment of prostate cancer with the 5α-reductase inhibitor turosteride in Dunning R3327 prostatic carcinoma in rats
✍ Zaccheo, Tiziana; Giudici, Donata; di Salle, Enrico 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 204 KB 👁 1 views

## Background: Turosteride, a selective 5alpha-reductase inhibitor, was reported to be effective in inhibiting the growth of established tumors in the dunning r3327 rat prostatic carcinoma model. we evaluated the preventive effect of turosteride when administered during the latency period in this p

Pharmacological and molecular evidence f
Pharmacological and molecular evidence for the expression of the two steroid 5α-reductase isozymes in normal and hyperplastic human prostatic cells in culture
✍ Berthaut, Isabelle; Mestayer, Chidi; Portois, Marie-Claire; Cussenot, Olivier; M 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 164 KB 👁 1 views

## BACKGROUND. Whereas the embryological development of the human prostate is clearly dependent on steroid 5␣-reductase (5␣-R) type 2 expression, the respective expression of the two known isoforms (types 1 and 2) of 5␣-R in the adult human prostate remains unclear. METHODS. 5␣-R isoform mRNA expr