Pseudomonas aeruginosa 142 and a presumed variant were grown axenically in chemostats on salicylate/benzoate or salicylate/glucose binary feeds. Each substrate was supplied at 2, 10, 50, 90, 98, or 100% of the total energy flux. Two experiments were also run with ternary mixtures using the same subs
Effects of suboptimal environmental conditions on immobilized bacteria growing in continuous culture
✍ Scribed by R. Diekmann; M. Naujoks; M. Gerdes-Kühn; D. C. Hempel
- Publisher
- Springer
- Year
- 1990
- Tongue
- English
- Weight
- 693 KB
- Volume
- 5
- Category
- Article
- ISSN
- 1615-7605
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✦ Synopsis
Two immobilized bacterial cultures with the ability to metabolize 6-amino-2-naphthalenesulfonic acid (6A2NS) and 2naphthalene-sulfonic acid (2NS) were investigated under suboptimal environmental growth conditions. The cultures were employed in continuously operated airiift loop-reactors. The physico-chemical growth parameters such as pH, temperature and dissolved oxygen concentration were varied. It was found that a decreasing growth rate in suboptimal conditions was compensated by increasing biomass concentration over a wide range. Operated continuously for more than 20 months, the 6A2NS-degrading system appeared to be reliable. After pH-shockloadings and long-term oxygen default, the immobilized microorganisms recovered almost immediately and stable operating conditions were achieved again within less than 48 h. These remarkable results were sustained with the 2NS degrading system.
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